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New single-cell sequencing method has the potential to boost efforts to find cancer-fighting drugs. Most cancerous tissue consists of rapidly dividing cells with a limited capacity for self-renewal, meaning that the bulk of cells stop reproducing after a certain number of divisions. However, cancer stem cells can replicate indefinitely, fuelling long-term cancer growth and driving relapse. Cancer stem cells that elude conventional treatments like chemotherapy are one of the reasons patients initially enter remission but relapse soon after. However, cancer stem cells are difficult to isolate and study because of their low abundance and similarity to other types of stem cell. This has hampered international research efforts to develop precision treatments that target malignant cells while sparing healthy ones. Researchers from EMBL and the Centre for Genomic Regulation CRG in Barcelona have overcome this problem by developing a method called MutaSeq, which can be used to distinguish between cancer stem cells, mature cancer cells, and otherwise healthy stem cells based on their genetics and gene expression patterns.
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